林麝源屎肠球菌LS170308株多重耐药基因岛PRI1分析

doi:10.11843/j.issn.0366-6964.2019.06.018

摘要/Abstract

摘要： 屎肠球菌作为条件致病菌在临床上报道的越来越多，主要引起宿主心内膜炎、败血症等，目前关于屎肠球菌的耐药性研究已日益突出，其原因主要在于该菌对β-内酰胺类药物天然耐药及对万古霉素等糖肽类抗生素耐药性不断增强，但该菌耐药性传播机制仍在进一步研究中。本研究拟描述1株林麝源屎肠球菌多重耐药基因岛的特征，显示出该基因岛在不同属细菌中的传播。采用单分子测序技术对这株林麝心源屎肠球菌进行从头测序，同时采用分子生物学工具对该基因岛进行分析。结果显示：这株屎肠球菌染色体上携带多个耐药基因，其中携带ANT（9）、ErmA的Tn554转座酶和携带AAC（6'）-le-APH（2″）-la的一对插入元件IS256共同构成的一个多重耐药基因岛来自金黄色葡萄球菌，该基因岛对大环内酯类、林可酰胺类及氨基糖苷类抗生素具有抗性。结果表明从死亡林麝内脏组织中分离得到1株屎肠球菌，该菌株携带有一个多重耐药基因组岛PRI1，该类型基因岛目前在屎肠球菌中并未有相关报道，同时这株菌来自野生动物，可为野生动物源细菌耐药性传播提供数据支持。

Abstract: Enterococcus faecium has been reported as a conditional pathogen in clinical practice, mainly causing endocarditis and sepsis in the host. At present, the research on drug resistance of E. faecium has become increasingly prominent, mainly because the bacteria are natural resistant to β-lactam and its resistant ability to glycopeptide antibiotics (such as vancomycin) are increasing, but the drug resistance transmission mechanism of this strain is still under study. The purpose of this study was to describe the characteristics of a multidrug resistance genomic island from E. faecium. Highlight the spread of the genomic island in different genera of bacteria. At the same time, it provides data that support for the spread of bacterial resistance in wild animals. Single-molecule sequencing technology (de nove) was used in this E. faecium, and the genomic island was analyzed using all kinds of molecular biology tools. Results were as follows:This strain of E. faecium carries multiple resistance genes on its chromosome; The Tn554 transposase carrying ANT(9) and ErmA and the pair of intercalating elements IS256 carrying AAC(6')-le-APH(2″)-la together constitute a multidrug resistance genomic island from Staphylococcus aureus. The genomic island is resistant to macrolides, lincosamides and aminoglycoside antibiotics. In conclusion, a strain of E. faecium was isolated from the visceral tissue of dead forest carp, which carries a multi-drug resistant genomic island PRI1. This genomic island is currently not reported in E. faecium. At the same time, this strain is derived from wild animals, so the resistance of wild animals has become more and more serious.

中图分类号:

龚永平, 陈珍容, 阴文奇, 文继峰, 易可可, 颜其贵. 林麝源屎肠球菌LS170308株多重耐药基因岛PRI1分析[J]. 畜牧兽医学报, 2019, 50(6): 1275-1283.

GONG Yongping, CHEN Zhenrong, YIN Wenqi, WEN Jifeng, YI Keke, YAN Qigui. Analysis of Multidrug-resistant Genomic Island PRI1 of Enterococcus faecium LS170308 from Moschus berezovskii[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(6): 1275-1283.

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